What Is the GI Map Test with Zonulin and Stool OMX
What Is the GI-MAP with Zonulin and Stool OMX Test?
The GI-MAP (GI Microbial Assay Plus) uses quantitative PCR (qPCR) DNA technology to detect and quantify microbes from a single stool sample. Unlike traditional stool cultures that can take weeks and often miss key pathogens, GI-MAP reveals the actual DNA of viruses, parasites, fungi, and bacteria—both good and bad.
What it screens for:
- Pathogenic bacteria (e.g., Salmonella, Shigella, C. diff)
- Opportunistic microbes and dysbiotic patterns
- Yeast/fungal overgrowth (e.g., Candida)
- Viruses and parasites
- Intestinal immune and inflammation markers
- Antibiotic resistance genes and virulence factors
This test is fast, accurate, and validated by FDA-approved molecular methods like the GPP assay from Luminex Corp .
Why Zonulin Matters: Leaky Gut in Real Time
Zonulin is a key marker for intestinal permeability—aka “leaky gut.” When zonulin levels are elevated, it means the tight junctions between your gut cells are loosening, allowing inflammatory molecules and microbes to slip into circulation.
High zonulin levels have been linked to:
- Autoimmune conditions
- Inflammatory bowel disease (IBD)
- Celiac disease
- Brain fog, fatigue, and mood disorders
By measuring zonulin directly, you get real-time insight into whether your gut barrier is intact—or letting toxins leak through.
🧠 This is essential information if you’re dealing with chronic inflammation, autoimmune symptoms, food sensitivities, or MCAS.
Introducing Stool OMX: Metabolomics for the Microbiome
The Stool OMX add-on is new for 2025 and represents a breakthrough in understanding gut function through metabolomics. It evaluates:
- 25 bile acids
- 9 microbial metabolites (short- and branched-chain fatty acids)
This matters because over 10% of your metabolome comes from gut microbes. If you’re not producing the right metabolites—or if bile acids are out of balance—it could be affecting your digestion, immune health, and even your mood .
Understanding Bile Acids: Beyond Fat Digestion
Bile acids do more than emulsify fats. They regulate immune function, blood sugar, inflammation, and even gut-brain signaling.
GI-MAP + OMX can detect:
- Bile acid diarrhea (seen in up to 30% of IBS-D patients)
- Malabsorption and microbial imbalances
- Conversion efficiency from primary to secondary bile acids (a critical gut-microbiome partnership)
This is clinical gold when addressing IBS, SIBO, gallbladder issues, or hormone detoxification.
SCFAs: Microbial Metabolites of Resilience
Short-chain fatty acids (SCFAs) like butyrate, acetate, and propionate reflect the health of your gut bacteria.
- Butyrate fuels your colon cells and protects against inflammation.
- Acetate crosses the blood-brain barrier and influences appetite regulation.
- Propionate supports blood sugar regulation and cholesterol balance.
These metabolites are essential for gut lining repair, immune modulation, and healthy brain chemistry .
Who Can Benefit from This Test?
This test is ideal for individuals struggling with:
- Bloating, constipation, diarrhea, or abdominal pain
- Food sensitivities or histamine intolerance
- Autoimmune symptoms
- Brain fog, anxiety, or fatigue
- Chronic skin issues
- Hormonal imbalances
- MCAS or Alpha-Gal Syndrome
Whether you’re a clinician or a health-savvy consumer, this test offers personalized, actionable insights.
Direct-to-Consumer Access via CanadaGIMap.com
The GI-MAP with Zonulin and OMX is now available through MyLabsForLife.com—no doctor’s visit required.
Benefits of ordering direct:
- At-home collection kits
- No insurance hassles
- Clinical-grade testing used by functional medicine practitioners worldwide
- Optional add-on: personalized consultation for interpreting your results
Take the First Step Toward Healing
Your gut is the command center of your health. If it’s inflamed, leaky, or out of balance, you’ll feel it in every system of your body. But healing is possible—when you start with clarity.
Order the GI-MAP with Zonulin and OMX today through MyLabsForLife.com
Easy at-home collection
Cutting-edge diagnostics
Actionable results
Optional expert guidance
Your microbiome is talking. This test helps you listen—and take action.
Health Disclaimer: It is recommended the reader of this site consult with a qualified healthcare provider of their choice when using any information obtained from this site, affiliate sites, and other online websites and blogs. Please consult your healthcare provider before making any healthcare decisions or for guidance about a specific medical condition.
References
- Camilleri M, Vijayvargiya P. The role of bile acids in chronic diarrhea. Am J Gastroenterol. 2020;115(10):1596-1603.
- Diagnostic Solutions Laboratory. GI-MAP Technical Information. https://www.diagnosticsolutionslab.com/tests/gi-map
- Khanna S, Tosh PK. A clinician’s primer on the role of the microbiome in human health and disease. Mayo Clin Proc. 2014;89(1):107-114.
- Petra AI, et al. Gut-microbiota-brain axis and its effect on neuropsychiatric disorders with suspected immune dysregulation. Clin Ther. 2015;37(5):984-995.
- Sturgeon C, Fasano A. Zonulin, a regulator of epithelial and endothelial barrier functions. Tissue Barriers. 2016;4(4):e1251384.
- Valdes AM, Walter J, Segal E, Spector TD. Role of the gut microbiota in nutrition and health. BMJ. 2018;361:k2179.
- Wang K, et al. Performance of a new molecular assay for the detection of gastrointestinal pathogens. J Clin Microbiol. 2020;58(12):e01739-20.
- Winston JA, Theriot CM. Diversification of host bile acids by members of the gut microbiota. Gut Microbes. 2020;11(2):158-171.